Deep tissue imaging at sub-micron resolution

Pryer lens

The Pryer lens is a new endoscopic objective compatible with common optical microscopes, especially modern Two-photon microscopes. An unconventional optical design combines a very small tip size to be minimally invasive with a large numerical aperture for high resolution.

This state-of-the-art objective allows for in vivo deep tissue access and visualization in difficult-to-reach regions such as the hippocampus. Image acquisition with subcellular spatial resolution at sub-second sampling rates becomes a reality.


The Pryer lens is a combined endoscopic objective and is compatible with any fluorescence microscopes, such as current Two-photon microscopes from Olympus and Nikon. This state-of-the-art objective allows for deep tissue access and visualization in anesthetized animals, awake animals with head fixation, and even chronic recordings.

It is composed of 7 lenses within its unique slim tip which makes it suitable for minimally invasive endoscopic imaging applications.

Magnification 70x
Numerical Aperture 0.7
Type Plan Fluorite NIR
Immersion medium Water
Correction Infinity / Zero cover glass thickness
Tip diameter 2.1 mm
Optical parameters
Working distance 380µm
Field of view 200µm
Transmission range 400nm-1080nm
  • Can be easily integrated into most  Two-photon microscopes that currently use Olympus or Nikon lenses
  • Excellent spatiotemporal resolution of <0.4 μm
  • Exceptionally slim objective tip diameter of 2.1 mm
  • Minimally invasive recordings from deep tissues such as deep brain regions (e.g. hippocampus)
  • Ideal for in vivo, acute imaging of awake and anesthetized animals
  • Provides access to any organ or tissue from the surface to deep structures

Pryer lens

The Pryer lens is compatible with all major types of objective mounts.

In vivo image recording from mouse hippocampus with the Pryer endoscopic objective

In vivo image recording from deep layers in mouse cortex with the Pryer endoscopic objective

A collection of z-stacks and 3D representations recorded with the Pryer lens is available here: Pryer_Lens_Video Examples

  • Neuroscience – enabling neuroscientists to visualize deep brain tissue such as the hippocampus. Imaging microglia in the brain and spinal cord. Measuring mast cell degranulation in the dura.
  • Immunology – allowing immunologists to record from spleen and lymph nodes through small lesions on the animal body.
  • Botany– enables botanists to spot on transplantation developments and other events such as pollination and fertilization.