JetBall–TFT

JetBall

Virtual Reality (VR) systems can be used with mice and rats to investigate brain functions during decision making. The JetBall-TFT is a worldwide unique product with a TFT surround monitor system and a spherical treadmill with optional operant devices. The JetBall, an air cushioned spherical treadmill allows a restrained animal to navigate in a virtual world. Brain function can be examined with electrophysiological, imaging and optogenetic methods while the animal performs behavioural tasks employing navigation, cognition, learning, or memory.

If you missed our VR landing page about the benefits of experimenting with VR, read more

  • TFT surround monitor system with 270°
  • JetBall air-cushion supported spherical treadmill
  • Large space for external setups, e.g. multi-photon microscopes or e-phys
  • High image contrast and luminance
  • Predefined experiments or create your own ones with our software
  • Data transfer system to combine JetBall data with external data.

“The virtual reality system from Phenosys allows us to perform stable electrophysiological recordings with glass electrodes and silicon probes from mice running on a jet ball. The combination of visual, olfactory and auditory stimuli combined with an online read-out of behavioural actions makes this system an ideal tool to study neuronal circuits in the operating brain. We have been successfully using this system to study gamma oscillations, place cells and spatial navigation in the hippocampus (Lasztoczi and Klausberger, Neuron 2014; Lasztoczi and Klausberger, Neuron 2016).”

Prof. Dr. Thomas Klausberger (MedUni Wien, Austria)

 

Lasztoczi B, Klausberger T. (2014) Layer-Specific GABAergic Control of Distinct Gamma Oscillations in the CA1 Hippocampus. Neuron, 81: 1126-1139 read more

Lasztoczi B, Klausberger T. (2016) Hippocampal Place Cells Couple to Three Different Gamma Oscillations during Place Field Traversal. Neuron, 91: 34–40 read more

 

Examples of neural dynamics during navigation and decision making in, e.g., a Corridor or T-Maze:

  • Intracellular dynamics and population activity of hippocampal place cells
  • Grid cell firing fields in virtual environments
  • Neocortical neural coding during decisions.

“Fuhrmann et al. identify a glutamatergic medial septal circuit that controls the initiation and velocity of locomotion. This circuit mediates the pre-motor initiation of theta oscillations and, via hippocampal disinhibition, underlies the locomotion speed dependence of hippocampal neuronal firing rates.”

Fuhrmann et al., 2015

 

Fuhrmann F. et al., 2015. Locomotion, Theta Oscillations, and the Speed-Correlated Firing of Hippocampal Neurons Are Controlled by a Medial Septal Glutamatergic Circuit. Neuron 86: 1–12 read more

Power
Power input voltage 100-240 V AC
Power consumption 850 W (maximal), 500 W (typical)
TFT Surround Monitor
270°, in octagonal arrangement with six 19”-TFT sub-units Top view
Height 44 cm
Weight 29.7 kg
Frequency 75 Hz
Resolution - display 1280(x3) x 1024 pixels
JetBall Spherical Treadmill, 20 cm for Mice
Ball Holder lenght 20 cm
Ball Holder width 20 cm
Ball Holder height 8 cm
Ball Holder height with ball (without air flow) 21 cm
Ball Holder weight 5.5 kg
Ball Holder material Aluminium
Ball diameter 20 cm
Ball material Polystyrene
JetBall Spherical Treadmill, 30 cm for Rats (<300g)
Ball Holder lenght 30.2 cm
Ball Holder width 30.2 cm
Ball Holder height 12.0 cm
Ball Holder height with ball (without air flow) 31.0 cm
Ball Holder weight 19.8 kg
Ball Holder material Aluminium
Ball diameter 30 cm
Ball material Polystyrene
Pressure Regulation
Flow meter 4-50 l/min, for experiments: 25 l/min
Pressure regulator 6.9 bar (maximal), for experiments: 2 bar
Sound pressure level 48-52 dBA (top of the ball, 1 cm distance from the ball, air flow 20 l/min)
Lick Sensor
Material (outer cover) Polyvinyl chloride
XY-Motion-Sensor
Resolution 200-5700 dpi
Max. speed Up to 4.19 m/s
Peristaltic Pump
Typical flow rate 0.45 ml/min
Movable Rack
Width 59.5 cm
Depth 62.0 cm
Height (without control monitor) 98.5 cm
CategoryOptionDescriptionIn Basic Package included
JetBall Basic Package
  • For mice, 20cm Spherical Treadmill System.
  • For rats (<300g), 30cm Spherical Treadmill System.

TFT Monitor Surround System 270°, Spherical Treadmill (Ball Holder, two balls), Dual XY-Motion Sensor, movable rack, computer (pre-installed: PhenoSoft Control, PhenoSoft Schedule, PhenoSoft VR, Virtual Corridor 2 m, control monitor, keyboard, computer mouse, power supply unit, pressure regulator system (compressor is not included).

Virtual Mazes
  • Endless Corridor
  • T-Maze
  • Y-Maze
  • Plus-Maze
  • Open Field 1 m

Each maze is available with mode for free or linear run.

Ball Holder
  • Ball Holder System 20cm, for mice.
  • Ball Holder System 30cm, for rats (<300 g).

Basic package includes one Ball Holder.
Also useable as a stand-alone product without a virtual reality system.

Extra Ball Holder
  • Ball Holder System 20cm, for mice.
  • Ball Holder System 30cm, for rats (<300 g).

For training.
Also available as a stand-alone product without a virtual reality system.

Ball
  • Ball 20cm for mice.
  • Ball 30cm for rats (<300 g).

Two balls are included in the Basic Package.

Extra Ball
  • Ball 20cm for mice.
  • Ball 30cm for rats (<300 g).
Positive Reward
  • Liquid Reward System, 20cm.
  • Liquid Reward System, 30cm.

Retractable: position is adjustable, servo-driven.
Lick Sensor: a drop of water is only delivered when the animal licks.

Negative Reward
  • Air Puff System, 20cm.
  • Air Puff System, 30cm.

Frontal air puff.

Whisker Stimulation
  • Whisker Stimulation System, 20cm.
  • Whisker Stimulation System, 30cm.

Air flow when animal is approaching virtual walls.
Twin system for left and right whiskers, position adjustable.

Brakes
  • Brake System, 20cm.
  • Brake System, 30cm.

To stop the spherical treadmill, e.g. at the end of a virtual corridor or after a wrong decision in a Y-maze

Acoustic
  • Acoustic System

E.g. to use a sound as a landmark.

Odour
  • Olfactometer

E.g. to combine an odour with a landmark.

Body Fixation
  • Head Holder for mice with 4 bars.

For optogenetics, imaging or electrophysiology.

Overview of the JetBall-TFT System

  • Surround Monitor with virtual reality scene
  • JetBall spherical treadmill: aluminium Ball Holder with polystyrene ball and the various operant modules
  • Four mounting rails on top of the surround monitor for, e.g., optional speakers.

JetBall with customized TFT surround monitor ring.

Double door configuration, partially opened.

JetBall Spherical Treadmill

Aluminium Ball Holder with polystyrene ball and the various operant modules (view from the top). The modules are highlighted in the following pictures.

Liquid Reward System

Mounted on the retractable operant device of the Ball Holder. A piezo-based lick sensor (Lick Sensor) is integrated into a cannula; there is an amplifier (black box with knob) with adjustable sensitivity.

Air Puff System

Mounted on the retractable operant device of the Ball Holder. It consists of a metal tube connected by plastic tubing to a valve. Tubing behind the valve connects to the flow meter and   the compressed air system.

Brake System

With two lateral and a frontal servo-driven brake.

Whisker Stimulation

Consisting of two metal tubes mounted on articulated arms that are attached to the Ball Holder. Plastic tubing connects to the valves of the compressed air system.

Dual XY-Motion Sensor

Two xy-sensors measure the rotation of the polystyrene ball in x, y and z (yaw, pitch, roll).

Body Fixation

Ball Holder (view from the side) with holder for fixing the mouse (on the right side).

Control Unit with Movable Rack

  • Control monitor
  • Keyboard and computer mouse
  • Pressure regulator system with flow meter and pressure regulator (second pressure regulator for air puff/whisker stimulation)
  • Computer
  • Power supply unit.

PhenoSoft Control – User Interface

Basic experimental control software used with all products by PhenoSys. Control of hardware components.

PhenoSoft Control – Log File

The log file containing the experimental data is written in a CSV format and is automatically saved.

PhenoSoft Schedule

Experimental schedules are defined with the help of state diagrams by using predefined activities from PhenoSoft.

Olympus

JetBall-TFT for mice (20cm ball) with a FVMPE-RS from Olympus.

Booth Olympus, FOM 2015.

Femtonics

JetBall-TFT for mice with a Femto2D microscope from the company Femtonics.

Customized solution for 2-photon microscope

TFT ring with double doors. The two central screens are foldable and allow easy access to the interior of the TFT-monitor ring.

TFT monitor ring with double doors and VR display. Fully opened.