Chan, K., et al. Induced Retinal Functional Alterations and Second-Order Neuron Plasticity in C57BL/6J Mice.

/ Publications / Chan, K., et al. Induced Retinal Functional Alterations and Second-Order Neuron Plasticity in C57BL/6J Mice.

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Chan, K., et al. Induced Retinal Functional Alterations and Second-Order Neuron Plasticity in C57BL/6J Mice.

Authors: Kore Chan; Mrinalini Hoon; Bikash R. Pattnaik; James N. Ver Hoeve; Brad Wahlgren; Shawna Gloe; Jeremy Williams; Brenna Wetherbee; Julie A. Kiland; Kara R. Vogel; Erwin Jansen; Gajja Salomons; Dana Walters; Jean-Baptiste Roullet; K Michael Gibson; Gillian J. McLellan

Publication: Investigative Ophthalmology & Visual Science, 61(2), 17, 2020 (Abstract)

DOI Link: https://doi.org/10.1167/iovs.61.2.17

Abstract

Vigabatrin (VGB) is an effective antiepileptic that increases concentrations of inhibitory γ-aminobutyric acid (GABA) by inhibiting GABA transaminase. Reports of VGB-associated visual field loss limit its clinical usefulness, and retinal toxicity studies in laboratory animals have yielded conflicting results.

Methods: We examined the functional and morphologic effects of VGB in C57BL/6J mice that received either VGB or saline IP from 10 to 18 weeks of age. Retinal structure and function were assessed in vivo by optical coherence tomography (OCT), ERG, and optomotor response. After euthanasia, retinas were processed for immunohistochemistry, and retinal GABA, and VGB quantified by mass spectrometry.