PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities

Genetically encoded calcium (Ca2+) indicators (GECIs) are essential tools for monitoring neuronal activity, but the performance of red fluorescent GECIs has remained limited. In particular, many red indicators are relatively dim, produce low signal-to-noise ratios and can undergo unwanted photoswitching when exposed to blue light, restricting their use in all-optical experiments that combine imaging with […]

Body weight is maintained through homeostatic mechanisms that balance energy intake and energy expenditure. To combat obesity, research has focused on activating thermogenesis, which accounts for 10%–15% of daily energy expenditure in humans but can expand to 30% with maximal activation (Ouellet et al., 2012). Adipocytes are central players in body weight maintenance and thermogenesis. There […]